Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

Al-Hindi, Rashad R ORCID: https://orcid.org/0000-0002-4674-617X, Teklemariam, Addisu D ORCID: https://orcid.org/0000-0002-0270-4568, Alharbi, Mona G, Alotibi, Ibrahim ORCID: https://orcid.org/0000-0001-5480-6294, Azhari, Sheren A, Qadri, Ishtiaq ORCID: https://orcid.org/0000-0003-4334-0585, Alamri, Turki ORCID: https://orcid.org/0000-0003-4382-4109, Harakeh, Steve ORCID: https://orcid.org/0000-0001-7512-8787, Applegate, Bruce M and Bhunia, Arun K ORCID: https://orcid.org/0000-0003-3640-1554 (2022) Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment. Biosensors, 12 (10). 905. ISSN 2079-6374

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Abstract

Foodborne microorganisms are an important cause of human illness worldwide. Two-thirds of human foodborne diseases are caused by bacterial pathogens throughout the globe, especially in developing nations. Despite enormous developments in conventional foodborne pathogen detection methods, progress is limited by the assay complexity and a prolonged time-to-result. The specificity and sensitivity of assays for live pathogen detection may also depend on the nature of the samples being analyzed and the immunological or molecular reagents used. Bacteriophage-based biosensors offer several benefits, including specificity to their host organism, the detection of only live pathogens, and resistance to extreme environmental factors such as organic solvents, high temperatures, and a wide pH range. Phage-based biosensors are receiving increasing attention owing to their high degree of accuracy, specificity, and reduced assay times. These characteristics, coupled with their abundant supply, make phages a novel bio-recognition molecule in assay development, including biosensors for the detection of foodborne bacterial pathogens to ensure food safety. This review provides comprehensive information about the different types of phage-based biosensor platforms, such as magnetoelastic sensors, quartz crystal microbalance, and electrochemical and surface plasmon resonance for the detection of several foodborne bacterial pathogens from various representative food matrices and environmental samples.