Effective alternative curing/clearance of biofilm associated and antibiotic resistant bacterial infections: a relevant probiotics model

Elijah, Jennifer Imade, Igere, Bright Esegbuyota and Butler, Jonathan ORCID: https://orcid.org/0000-0002-1323-8611 (2025) Effective alternative curing/clearance of biofilm associated and antibiotic resistant bacterial infections: a relevant probiotics model. Biomedical Analysis, 2 (1). pp. 8-15. ISSN 2950435X

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Abstract

Diverse bio-inhibitory agents have been applied as control model for microbial proliferation with specific relevance/capability in effectively balancing various nexus microbiome. However, with recent reports on growing resistance mechanism and health concerns associated with bacterial (Pseudomonas aeruginosa) infections, an alternative model for treatment becomes imminent. One suggestive and alternative bio-inhibitory agent of relevance is probiotics which was prepared from the Genus Bacillus. This study determine the effective curing/clearing capacity of probiotics; a model in wound infection control and biofilm inhibition. Briefly, biofilm producing P. aeruginosa were used to challenge surfaces as infectious-strain, while surface challenge test was done to determine probiotics effectiveness as surface cleaner. In vitro antibacterial susceptibility testing using probiotics was done on 21 selected biofilm producing P. aeruginosa of clinical and environmental origin. Time kill assays was done at different time interval (0–24 h) and different dilutions (1:10 and 1:20) on biofilm producing strains with probiotics. In addition, probiotics was used as treatment for infected wound dressing to determine its effectiveness. Following statistical evaluations, the surface challenge test showed promising reports with notable probiotics inhibitory properties on tested strains as well as susceptibility testing. Also a reduction/biofilm clearance was observed especially for commercially obtained probiotics used for wound dressing with significant indications. From the forgoing, it is suggested that probiotics are model alternative therapeutic strategic agents for controlling/removing resistant potential pathogens. It may also be a model alternative surface cleaner, surface decontaminant, diverse microbiome control agent, removal of biofilm and treatment of recalcitrant wound infection pathogens with little or no identified side effect on both humans and environment.