Multiplex antibiotic susceptibility testing of urinary tract infections using an electrochemical lab-on-a-chip

Crane, Benjamin, Iles, Alex ORCID: https://orcid.org/0000-0003-0837-6160, Banks, Craig E ORCID: https://orcid.org/0000-0002-0756-9764, Rashid, Mamun ORCID: https://orcid.org/0000-0002-9769-2431, Linton, Patricia E ORCID: https://orcid.org/0000-0002-1106-4988 and Shaw, Kirsty J ORCID: https://orcid.org/0000-0001-9241-4195 (2024) Multiplex antibiotic susceptibility testing of urinary tract infections using an electrochemical lab-on-a-chip. Biomedical Microdevices, 26 (3). 35. ISSN 1387-2176

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Abstract

Urinary tract infections (UTIs) represent the most prevalent type of outpatient infection, with significant adverse health and economic burdens. Current culture-based antibiotic susceptibility testing can take up to 72 h resulting in ineffective prescription of broad-spectrum antibiotics, poor clinical outcomes and development of further antibiotic resistance. We report an electrochemical lab-on-a-chip (LOC) for testing samples against seven clinically-relevant antibiotics. The LOC contained eight chambers, each housing an antibiotic-loaded hydrogel (cephalexin, ceftriaxone, colistin, gentamicin, piperacillin, trimethoprim, vancomycin) or antibiotic-free control, alongside a resazurin bulk-modified screen-printed electrode for electrochemical detection of metabolically active bacteria using differential pulse voltammetry. Antibiotic susceptibility in simulated UTI samples or donated human urine with either Escherichia coli or Klebsiella pneumoniae could be established within 85 min. Incorporating electrochemical detection onto a LOC provides an inexpensive, simple method for the sensitive determination of antibiotic susceptibility that is significantly faster than using a culture-based approach.