Umbelliferone prevents isoproterenol-induced myocardial injury by upregulating Nrf2/HO-1 signaling, and attenuating oxidative stress, inflammation, and cell death in rats

Althunibat, Osama Y ORCID: https://orcid.org/0000-0002-3406-9634, Abduh, Maisa Siddiq, Abukhalil, Mohammad H, Aladaileh, Saleem H, Hanieh, Hamza and Mahmoud, Ayman M ORCID: https://orcid.org/0000-0003-0279-6500 (2022) Umbelliferone prevents isoproterenol-induced myocardial injury by upregulating Nrf2/HO-1 signaling, and attenuating oxidative stress, inflammation, and cell death in rats. Biomedicine and Pharmacotherapy, 149. 112900. ISSN 0753-3322

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Official URL: http://dx.doi.org/10.1016/j.biopha.2022.112900

Abstract

The role of oxidative injury and inflammatory response in cardiovascular diseases and heart failure has been well-acknowledged. This study evaluated the protective effect of umbelliferone (UMB), a coumarin with promising radical scavenging and anti-inflammatory activities, on myocardial injury induced by isoproterenol (ISO) in rats. Rats received 50 mg/kg UMB orally for 14 days and 85 mg/kg ISO twice at an interval of 24 h. Administration of ISO elevated serum troponin I, creatine kinase-MB and lactate dehydrogenase, and caused histopathological alterations, including degeneration, fatty vacuolation, myolysis, and atrophy of myocardial fibers. Malondialdehyde (MDA), nitric oxide (NO), nuclear factor-kappaB (NF-κB) p65, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β were increased, whereas reduced glutathione (GSH), superoxide dismutase (SOD), and catalase were decreased in ISO-administered rats. UMB effectively ameliorated myocardial injury, alleviated cardiac function markers, MDA, NO, NF-κB p65, and the inflammatory mediators, and enhanced cellular antioxidants. Bax, caspase-3, and 8-OHdG were decreased, and Bcl-2 was increased in ISO-administered rats treated with UMB. In addition, UMB upregulated nuclear factor-erythroid factor 2-related factor 2 (Nrf2) and heme oxygenase (HO)-1 in the heart of ISO-administered rats. In conclusion, UMB can protect the myocardium from oxidative injury, inflammatory response, and cell death induced by ISO by upregulating Nrf2/HO-1 signaling and antioxidants.

Item Type:	Article (Article)
Peer-reviewed:	Yes
Date Deposited:	16 May 2024 12:33
Publisher:	Elsevier BV
Additional Information:	This is an open access article which first appeared in Biomedicine and Pharmacotherapy, published by Elsevier
Divisions:	Faculties > Science and Engineering
Subject terms:	Animals, Rats, Inflammation, Isoproterenol, Umbelliferones, NF-kappa B, Antioxidants, Cell Death, Oxidative Stress, NF-E2-Related Factor 2, 7-Hydroxy coumarin, Antioxidants, Inflammation, Isoproterenol, Myocardial injury, Oxidative stress, Animals, Rats, Antioxidants, Cell Death, Inflammation, Isoproterenol, NF-E2-Related Factor 2, NF-kappa B, Oxidative Stress, Umbelliferones, 1115 Pharmacology and Pharmaceutical Sciences, Oncology & Carcinogenesis
Data Access Statement:	The manuscript contains all data supporting the reported results.
URI:	https://e-space.mmu.ac.uk/id/eprint/634656
DOI:	https://doi.org/10.1016/j.biopha.2022.112900
ISSN	0753-3322
e-ISSN	1950-6007

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