Molybdenum Disulphide Surfaces to Reduce Staphylococcus aureus and Pseudomonas aeruginosa Biofilm Formation

Amin, Mohsin, Rowley-Neale, Samuel James ORCID: https://orcid.org/0000-0002-3741-4050, Shalamanova, Liliana, Lynch, Stephen ORCID: https://orcid.org/0000-0002-4183-5122, Wilson-Nieuwenhuis, Joels, El Mohtadi, Mohamed, Banks, Craig E ORCID: https://orcid.org/0000-0002-0756-9764 and Whitehead, Kathryn A (2020) Molybdenum Disulphide Surfaces to Reduce Staphylococcus aureus and Pseudomonas aeruginosa Biofilm Formation. ACS applied materials & interfaces, 12 (18). pp. 21057-21069. ISSN 1944-8244

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Abstract

The reduction of bacteria and biofilm formation is important when designing surfaces for use in industry. Molybdenum disulphide surfaces (MoS2SUR) were produced using MoS2 particle (MoS2PAR) sizes of 90 nm 2 µm and 6 µm containing MoS2PAR concentrations of 5%, 10%, 15% and 20%. These were tested to determine the efficacy of the MoS2SUR to impede bacterial retention and biofilm formation of two different types of bacteria, Staphylococcus aureus and Pseudomonas aeruginosa. The MoS2SUR were characterised using Fourier Transform InfraRed Spectroscopy, Ion Coupled Plasma Atomic Emission Spectroscopy, Scanning Electron Microscopy, Optical Profilometry and Water Contact Angles. The MoS2SUR made with the smaller 90 nm MoS2PAR sizes demonstrated smaller topographical shaped features. As the size of the incorporated MoS2PAR increased, the MoS2SUR demonstrated wider surface features, and they were less wettable. The increase in MoS2PAR concentration within the MoS2SUR groups did not affect the surface topography but did increase wettability. However, the increase in MoS2PAR size increased both the surface topography and wettability. The MoS2SUR with the smaller topographical shaped features, influenced the retention of the S. aureus bacteria. Increased MoS2SUR topography and wettability resulted in the greatest reduction in bacterial retention and the bacteria became more heterogeneously dispersed and less clustered across the surfaces. The surfaces that exhibited decreased bacterial retention (largest particle sizes, largest features, greatest roughness, most wettable) resulted in decreased biofilm formation. Cytotoxicity testing of the surface using cell viability demonstrated that the MoS2SUR were not toxic against HK-2 cells at MoS2PAR sizes of 90 nm and 2 µm. This work demonstrated that individual surfaces variables (MoS2SUR topographic shape and roughness, MoS2PAR size and concentration) decreased bacterial loading on the surfaces, which then decreased biofilm formation. By optimising MoS2SUR properties, it was possible to impede bacterial retention and subsequent biofilm formation.