Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H2O2 biosensor applications

Palanisamy, S, Velusamy, V, Chen, SW, Yang, TCK, Balu, S and Banks, CE (2019) Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H2O2 biosensor applications. Carbohydrate Polymers, 204. pp. 152-160. ISSN 0144-8617

Preview

Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (2MB) | Preview

Abstract

© 2018 Elsevier Ltd. In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H2O2biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various physio-chemical methods have applied for the characterization of RGO-CMF composite. Cyclic voltammetry results revealed that the hemin/RGO-CMF composite shows a better redox electrochemical behavior than hemin/RGO and hemin/GO-CMF. Under optimized conditions, the hemin/RGO-CMF composite exhibit a linear response to H2O2in the concentration range from 0.06 to 540.6 μM with the lower detection limit of 16 nM. The sensor also can able to detect the H2O2in commercial contact lens solution and milk samples with functional recovery, which authenticates the potential ability in practical sensors.