A pilot study investigating the influence of glucagon-like peptide-1 receptor single nucleotide polymorphisms on gastric emptying rate in Caucasian men

Yau, Mo Wah, McLaughlin, John, Maughan, Ronald, Gilmore, William, Ashworth, Jason ORCID: https://orcid.org/0000-0001-7045-7899 and Evans, Gethin ORCID: https://orcid.org/0000-0001-7530-1164 (2018) A pilot study investigating the influence of glucagon-like peptide-1 receptor single nucleotide polymorphisms on gastric emptying rate in Caucasian men. Frontiers in Physiology, 9. ISSN 1664-042X

Preview

Published Version Available under License Creative Commons Attribution. Download (234kB) | Preview

Abstract

Gastric emptying rate in humans is subject to large individual variability, but previous research on the influence of genetics is scarce. Variation in the glucagon-like peptide-1 receptor (GLP1R) gene is a plausible candidate gene to partially explain the high variance. This study aimed to investigate the influence of genetic variation in the GLP1R gene on gastric emptying rate of a glucose solution in humans. Forty eight healthy Caucasian males took part in this investigation. Gastric emptying rate of a 6% glucose solution was assessed using the 13C breath test method and a venous blood sample was obtained from each participant. Participants were genotyped for twenty-seven Tag single nucleotide polymorphisms (SNPs) in the GLP1R locus using Sequenom MassARRAY iPLEX GOLD analysis and MALDI-TOF mass spectrometry. The time at which maximal emptying rate occurred (Tlag) was faster in participants with the CC genotype than in TT and TC genotypes for SNP rs742764: (Median (quartiles) CC, 35 (30-36) min vs. TT, 43 (39-46) min and TC, 41 (39-45) min; P < 0.01). Tlag was also slower in participants with the AA genotype compared to the TT and TA genotypes for SNP rs2254336: (AA, 43 (39-49) min vs. TT, 36 (34-41) min and TA, 39 (35-42) min; P < 0.05). Analysis by phenotype also showed differences in half-emptying time (T½) and Tlag for SNPs rs9283907, rs2268657 and rs2254336. Several neighbouring Tag SNPs within the GLP1R gene were found to be associated with gastric emptying rate, and should be further investigated.