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    Stromal derived factor-1 exerts differential regulation on distinct cortical cell populations in vitro

    Pritchett, J, Wright, C, Zeef, L and Nadarajah, B (2007) Stromal derived factor-1 exerts differential regulation on distinct cortical cell populations in vitro. BMC Developmental Biology, 7. ISSN 1471-213X

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    Abstract

    Background. Stromal derived factor (SDF-1), an alpha chemokine, is a widely known chemoattractant in the immune system. A growing body of evidence now suggests multiple regulatory roles for SDF-1 in the developing nervous system. Results. To investigate the role of SDF-1 signaling in the growth and differentiation of cortical cells, we performed numerous in vitro experiments, including gene chip and quantitative RT-PCR analysis. Using SDF-1 medium and AMD3100, a receptor antagonist, we demonstrate that the chemokine signaling regulates key events during early cortical development. First, SDF-1 signaling maintains cortical progenitors in proliferation, possibly through a mechanism involving connexin 43 mediated intercellular coupling. Second, SDF-1 signaling upregulates the differentiation of cortical GABAergic neurons, independent of sonic signaling pathway. Third, SDF-1 enables the elongation and branching of axons of cortical glutamatergic neurons. Finally, cortical cultures derived from CXCR4-/- mutants show a close parallel to AMD3100 treatment with reduced cell proliferation and differentiation of GABAergic neurons. Conclusion. Results from this study show that SDF-1 regulates distinct cortical cell populations in vitro. © 2007 Pritchett et al; licensee BioMed Central Ltd.

    Item Type: Article
    Peer-reviewed: Yes
    Date Deposited: 18 May 2017 08:01
    Publisher: BioMed Central
    Additional Information: This is an Open Access article published in BMC Developmental Biology, published by BioMed Central, copyright The Author(s).
    Divisions: Faculties > Science and Engineering
    Subject terms: Cerebral Cortex, Neurons, Axons, Cells, Cultured, Animals, Mice, Rats, Receptors, CXCR4, Chemokines, CXC, Culture Media, Conditioned, Enzyme-Linked Immunosorbent Assay, Oligonucleotide Array Sequence Analysis, Immunohistochemistry, Reverse Transcriptase Polymerase Chain Reaction, Cell Differentiation, Cell Proliferation, Gene Expression Regulation, Developmental, Mutation, Chemokine CXCL12, Animals, Axons, Cell Differentiation, Cell Proliferation, Cells, Cultured, Cerebral Cortex, Chemokine CXCL12, Chemokines, CXC, Culture Media, Conditioned, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Developmental, Immunohistochemistry, Mice, Mutation, Neurons, Oligonucleotide Array Sequence Analysis, Rats, Receptors, CXCR4, Reverse Transcriptase Polymerase Chain Reaction, 06 Biological Sciences, 11 Medical And Health Sciences, Developmental Biology
    URI: https://e-space.mmu.ac.uk/id/eprint/618438
    DOI: https://doi.org/10.1186/1471-213X-7-31
    ISSN 1471-213X

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