Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

Mosley, O, Melling, L, Tarn, MD, Kemp, C, Esfahani, MMN, Pamme, N and Shaw, KJ (2016) Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 09 October 2016 - 13 October 2016, Dublin, Ireland.

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Abstract

We present a sample introduction interface that allows direct on-chip processing of crude stool samples for the detection of Helicobacter pylori (H. pylori). Efficient cell lysis and DNA extraction were achieved using stored reagents, reconstituted upon sample addition. Clinical stool samples were analysed using the device and successful amplification of a H. pylori specific target was achieved.