e-space
Manchester Metropolitan University's Research Repository

    Extensive developmental and metabolic alterations in cybrids nicotiana tabacum (+ hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility

    Zubko, Mikhajlo K., Zubko, Elena I., Ruban, Alexander V., Adler, Klaus, Mock, Hans-Peter, Misera, Simon, Gleba, Yuri Yu and Grimm, Bernhard (2001) Extensive developmental and metabolic alterations in cybrids nicotiana tabacum (+ hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility. The Plant Journal, 25 (6). pp. 627-639. ISSN 1365-313X

    File not available for download.

    Abstract

    The genetic basis of multiple phenotypic alterations was studied in cell-engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo-cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In 'green flowers' (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome–genome incompatibility, whereas other features are likely to be due to nucleo-mitochondrial incompatibilities.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    0Downloads
    6 month trend
    1,836Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record