Manchester Metropolitan University's Research Repository

    Depot-specific effects of fatty acids on lipid accumulation in children’s adipocytes

    Sabin, Matthew A., Crowne, Elizabeth, Stewart, Claire E., Hunt, Linda P., Turner, Stephen J. and Welsh, Gavin (2007) Depot-specific effects of fatty acids on lipid accumulation in children’s adipocytes. Biochemical and Biophysical Research Communications, 361 (2). pp. 356-361. ISSN 1090-2104

    File not available for download.


    Circulating concentrations of fatty acids are elevated in obesity, although their effect on regional fat deposition is relatively unexplored. With the increasing prevalence of childhood obesity, we aimed to investigate whether saturated and unsaturated fatty acids lead to differential lipid accumulation (LA) in children’s subcutaneous and visceral adipocytes. To examine this, subcutaneous and peri-nephric pre-adipocytes, isolated from fat biopsies from 6 pre-pubertal children, were differentiated in vitro before being exposed to palmitate and/or oleate for 24 h. Lipid accumulation was then quantified by nile red staining. Palmitate significantly increased LA in visceral adipocytes at all doses 188 μM (e.g. Palmitate 750 μM: +30.0%[8.2]; p < 0.01), whilst only a dose of 375 μM led to a significant, but smaller, increase in LA in subcutaneous adipocytes (Palmitate 375 μM: +13.0%[4.3]; p = 0.02). In contrast, oleate significantly increased LA in subcutaneous (Oleate 1000 μM: +36.3%[14.0]; p = 0.01), but not visceral (Oleate 1000 μM: +16.2%[9.6]; p = 0.25) adipocytes. These data suggest that saturated and unsaturated fatty acids may exert depot-specific effects on lipid accumulation.

    Impact and Reach


    Activity Overview
    6 month trend
    6 month trend

    Additional statistics for this dataset are available via IRStats2.


    Repository staff only

    Edit record Edit record