e-space
Manchester Metropolitan University's Research Repository

    Effect of dextranase and protease enzymes on aggregation of Streptococcus mutans: colorimetric and electron microscopic studies

    Graves, W. and Verran, Joanna (1984) Effect of dextranase and protease enzymes on aggregation of Streptococcus mutans: colorimetric and electron microscopic studies. Microbios, 40 (161-2). pp. 145-152. ISSN 0026-2633

    File not available for download.

    Abstract

    Sucrose-induced aggregation of Streptococcus mutans was ascertained by measuring the decrease in optical density of a cell suspension after 20 h incubation. The optimum sucrose concentration for the aggregation assay was 5% w/v, at an incubation temperature of 37 degrees C. Dextranase and protease enzymes were added to the suspensions to determine their effects on aggregation. Dextranase treatment decreased aggregation. Electron microscopic studies revealed a reduction in the amount of a diffuse coat which surrounded cells in the absence of the enzyme but in the presence of sucrose, and a degradative effect on fibrous extracellular material. Protease treatment of suspensions produced an increase in optical density; cell aggregates were not apparent. Electron microscopic studies revealed extensive cell damage and lysis. The success of these enzymes in decreasing aggregation would indicate a role in both caries and plaque control in vivo.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    0Downloads
    6 month trend
    279Hits

    Additional statistics for this dataset are available via IRStats2.

    Repository staff only

    Edit record Edit record