A systematic review for non-targeted analysis of per- and polyfluoroalkyl substances (PFAS)

Megson, David ORCID: https://orcid.org/0000-0002-8881-3860, Bruce-Vanderpuije, Pennante, Idowu, Ifeoluwa Grace, Ekpe, Okon Dominic and Sandau, Courtney D (2025) A systematic review for non-targeted analysis of per- and polyfluoroalkyl substances (PFAS). Science of The Total Environment, 960. 178240. ISSN 0048-9697

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Abstract

This review follows the PRISMA guidelines to provide a systematic review of 115 peer reviewed articles that used non-targeted analysis (NTA) methods to detect per- and polyfluoroalkylated substances (PFAS). This literature highlights the significant positive impact of NTA in understanding PFAS in the environment. Within the literature a geographical bias exists, with most NTA studies (∼60 %) conducted in the United States and China. Future studies in other regions (such as South America and Africa) are needed to gain a more global understanding. More research is required in marine environments and the atmosphere, as current studies focus mainly on freshwater, groundwater, soil, and sediments. The majority of studies focus on measuring PFAS in the environment, rather than in commercial products (with the exception of AFFF). Non-lethal blood sampling has been successful for NTA in humans and wildlife, but additional biomonitoring studies are required on exposed cohorts to understand health risks and PFAS biotransformation pathways. NTA methods mostly use liquid chromatography and negative ionisation, which biases the literature towards the detection of specific PFAS. Despite improvements in data reporting and quality assurance and control (QA/QC) procedures, factors such as false negative and false positive rates are often overlooked, and many NTA workflows remain highly subjective. Perfluoroalkyl carboxylic acids (PFCAs) and perfluoroalkyl sulfonic acids (PFSAs) are the most detected PFAS classes, identified in over 80 % of NTA studies, and are common in routine monitoring. However, our review identified >1000 PFAS from a total of 382 different PFAS classes, with over 300 classes found in fewer than 5 % of studies. This highlights the variety of different PFAS present in the environment, and the limitations of relying solely on targeted methods. Future monitoring programs and regulations would benefit from considering NTA methods to provide more comprehensive information on PFAS present in the environment.