Passive mechanical properties of adipose tissue and skeletal muscle from C57BL/6J mice

Cesanelli, L, Minderis, P ORCID: https://orcid.org/0000-0001-9522-6050, Degens, H ORCID: https://orcid.org/0000-0001-7399-4841 and Satkunskiene, D (2024) Passive mechanical properties of adipose tissue and skeletal muscle from C57BL/6J mice. Journal of the mechanical behavior of biomedical materials, 155. 106576. ISSN 1751-6161

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Official URL: http://dx.doi.org/10.1016/j.jmbbm.2024.106576

Abstract

Skeletal muscle and adipose tissue are characterized by unique structural features finely tuned to meet specific functional demands. In this study, we investigated the passive mechanical properties of soleus (SOL), extensor digitorum longus (EDL) and diaphragm (DIA) muscles, as well as subcutaneous (SAT), visceral (VAT) and brown (BAT) adipose tissues from 13 C57BL/6J mice. Thereto, alongside stress-relaxation assessments we subjected isolated muscles and adipose tissues (ATs) to force-extension tests up to 10% and 30% of their optimal length, respectively. Peak passive stress was highest in the DIA, followed by the SOL and lowest in the EDL (p < 0.05). SOL displayed also the highest Young's modulus and hysteresis among muscles (p < 0.05). BAT demonstrated highest peak passive stress and Young's modulus followed by VAT (p < 0.05), while SAT showed the highest hysteresis (p < 0.05). When comparing data across all six biological specimens at fixed passive force intervals (i.e., 20-40 and 50-70 mN), skeletal muscles exhibited significantly higher peak stresses and strains than ATs (p < 0.05). Young's modulus was higher in skeletal muscles than in ATs (p < 0.05). Muscle specimens exhibited slower force relaxation in the first phase compared to ATs (p < 0.05), while there was no significant difference in behavior between muscles and AT in the second phase of relaxation. The study revealed distinctive mechanical behaviors specific to different tissues, and even between different muscles and ATs. These variations in mechanical properties are likely such to optimize the specific functions performed by each biological tissue.

Item Type:	Article (Article)
Peer-reviewed:	Yes
Date Deposited:	28 May 2024 14:44
Publisher:	Elsevier BV
Additional Information:	© 2024. This accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
Divisions:	Faculties > Science and Engineering
Subject terms:	Muscle, Skeletal, Adipose Tissue, Animals, Mice, Inbred C57BL, Mice, Materials Testing, Stress, Mechanical, Male, Elastic Modulus, Mechanical Phenomena, Biomechanical Phenomena, Mechanical Tests, Adipocytes, Biomechanics, Extra cellular matrix, Mechanobiology, Muscle mechanics, Animals, Mice, Inbred C57BL, Mice, Muscle, Skeletal, Biomechanical Phenomena, Adipose Tissue, Mechanical Phenomena, Male, Stress, Mechanical, Elastic Modulus, Mechanical Tests, Materials Testing, 0903 Biomedical Engineering, 0912 Materials Engineering, 0913 Mechanical Engineering, Biomedical Engineering
Data Access Statement:	Data will be made available on request.
URI:	https://e-space.mmu.ac.uk/id/eprint/634764
DOI:	https://doi.org/10.1016/j.jmbbm.2024.106576
ISSN	1751-6161
e-ISSN	1878-0180

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