e-space
Manchester Metropolitan University's Research Repository

    Resveratrol activates antioxidant protective mechanisms in cellular models of Alzheimer’s disease inflammation

    Bartra, Clara ORCID logoORCID: https://orcid.org/0000-0001-7426-0751, Yuan, Yi, Vuraić, Kristijan, Valdés-Quiroz, Haydeé, Garcia-Baucells, Pau ORCID logoORCID: https://orcid.org/0009-0009-6784-8114, Slevin, Mark ORCID logoORCID: https://orcid.org/0000-0003-3767-4861, Pastorello, Ylenia ORCID logoORCID: https://orcid.org/0009-0004-3833-0184, Suñol, Cristina and Sanfeliu, Coral ORCID logoORCID: https://orcid.org/0000-0002-9004-1206 (2024) Resveratrol activates antioxidant protective mechanisms in cellular models of Alzheimer’s disease inflammation. Antioxidants, 13 (2). 177. ISSN 2076-3921

    [img]
    Preview
    Published Version
    Available under License Creative Commons Attribution.

    Download (6MB) | Preview

    Abstract

    Resveratrol is a natural phenolic compound with known benefits against neurodegeneration. We analyzed in vitro the protective mechanisms of resveratrol against the proinflammatory monomeric C-reactive protein (mCRP). mCRP increases the risk of AD after stroke and we previously demonstrated that intracerebral mCRP induces AD-like dementia in mice. Here, we used BV2 microglia treated with mCRP for 24 h in the presence or absence of resveratrol. Cells and conditioned media were collected for analysis. Lipopolysaccharide (LPS) has also been implicated in AD progression and so LPS was used as a resveratrol-sensitive reference agent. mCRP at the concentration of 50 µg/mL activated the nitric oxide pathway and the NLRP3 inflammasome pathway. Furthermore, mCRP induced cyclooxygenase-2 and the release of proinflammatory cytokines. Resveratrol effectively inhibited these changes and increased the expression of the antioxidant enzyme genes Cat and Sod2. As central mechanisms of defense, resveratrol activated the hub genes Sirt1 and Nfe2l2 and inhibited the nuclear translocation of the signal transducer NF-ĸB. Proinflammatory changes induced by mCRP in primary mixed glial cultures were also protected by resveratrol. This work provides a mechanistic insight into the protective benefits of resveratrol in preventing the risk of AD induced by proinflammatory agents.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    104Downloads
    6 month trend
    149Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record