e-space
Manchester Metropolitan University's Research Repository

    Modelling Skeletal Muscle Ageing and Repair In Vitro

    Tarum, Janelle, Degens, Hans ORCID logoORCID: https://orcid.org/0000-0001-7399-4841, Turner, Mark D, Stewart, Claire, Sale, Craig ORCID logoORCID: https://orcid.org/0000-0002-5816-4169 and Santos, Lívia (2023) Modelling Skeletal Muscle Ageing and Repair In Vitro. Journal of Tissue Engineering and Regenerative Medicine, 2023. pp. 1-15. ISSN 1932-7005

    [img]
    Preview
    Published Version
    Available under License Creative Commons Attribution.

    Download (4MB) | Preview

    Abstract

    Healthy skeletal muscle can regenerate after ischaemic, mechanical, or toxin-induced injury, but ageing impairs that regeneration potential. This has been largely attributed to dysfunctional satellite cells and reduced myogenic capacity. Understanding which signalling pathways are associated with reduced myogenesis and impaired muscle regeneration can provide valuable information about the mechanisms driving muscle ageing and prompt the development of new therapies. To investigate this, we developed a high-throughput in vitro model to assess muscle regeneration in chemically injured C2C12 and human myotube-derived young and aged myoblast cultures. We observed a reduced regeneration capacity of aged cells, as indicated by an attenuated recovery towards preinjury myotube size and myogenic fusion index at the end of the regeneration period, in comparison with younger muscle cells that were fully recovered. RNA-sequencing data showed significant enrichment of KEGG signalling pathways, PI3K-Akt, and downregulation of GO processes associated with muscle development, differentiation, and contraction in aged but not in young muscle cells. Data presented here suggest that repair in response to in vitro injury is impaired in aged vs. young muscle cells. Our study establishes a framework that enables further understanding of the factors underlying impaired muscle regeneration in older age.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    313Downloads
    6 month trend
    71Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record