e-space
Manchester Metropolitan University's Research Repository

    Electrochemical and thermal detection of allergenic substance lysozyme with molecularly imprinted nanoparticles

    Singla, Pankaj, Kaur, Sarbjeet, Jamieson, Oliver, Dann, Amy, Garg, Saweta, Mahon, Clare, Crapnell, Robert D ORCID logoORCID: https://orcid.org/0000-0002-8701-3933, Banks, Craig E ORCID logoORCID: https://orcid.org/0000-0002-0756-9764, Kaur, Inderpreet and Peeters, Marloes (2023) Electrochemical and thermal detection of allergenic substance lysozyme with molecularly imprinted nanoparticles. Analytical and Bioanalytical Chemistry. ISSN 1618-2642

    [img]
    Preview
    Published Version
    Available under License Creative Commons Attribution.

    Download (2MB) | Preview

    Abstract

    Lysozyme (LYZ) is a small cationic protein which is widely used for medical treatment and in the food industry to act as an anti-bacterial agent; however, it can trigger allergic reactions. In this study, high-affinity molecularly imprinted nanoparticles (nanoMIPs) were synthesized for LYZ using a solid-phase approach. The produced nanoMIPs were electrografted to screen-printed electrodes (SPEs), disposable electrodes with high commercial potential, to enable electrochemical and thermal sensing. Electrochemical impedance spectroscopy (EIS) facilitated fast measurement (5–10 min) and is able to determine trace levels of LYZ (pM) and can discriminate between LYZ and structurally similar proteins (bovine serum albumin, troponin-I). In tandem, thermal analysis was conducted with the heat transfer method (HTM), which is based on monitoring the heat transfer resistance at the solid–liquid interface of the functionalized SPE. HTM as detection technique guaranteed trace-level (fM) detection of LYZ but needed longer analysis time compared to EIS measurement (30 min vs 5–10 min). Considering the versatility of the nanoMIPs which can be adapted to virtually any target of interest, these low-cost point-of-care sensors hold great potential to improve food safety. Graphical Abstract: [Figure not available: see fulltext.].

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    52Downloads
    6 month trend
    43Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record