e-space
Manchester Metropolitan University's Research Repository

    Age‐related changes to the satellite cell niche are associated with reduced activation following exercise

    Nederveen, Joshua P, Joanisse, Sophie ORCID logoORCID: https://orcid.org/0000-0001-9983-9401, Thomas, Aaron CQ, Snijders, Tim, Manta, Katherine, Bell, Kirsten E, Phillips, Stuart M, Kumbhare, Dinesh and Parise, Gianni (2020) Age‐related changes to the satellite cell niche are associated with reduced activation following exercise. The FASEB Journal, 34 (7). pp. 8975-8989. ISSN 0892-6638

    [img]
    Preview
    Accepted Version
    Download (988kB) | Preview

    Abstract

    Skeletal muscle satellite cell (SC) function and responsiveness is regulated, in part, through interactions within the niche, in which they reside. Evidence suggests that structural changes occur in the SC niche as a function of aging. In the present study, we investigated the impact of aging on SC niche properties. Muscle biopsies were obtained from the vastus lateralis of healthy young (YM; 21 ± 1 yr; n = 10) and older men (OM; 68 ± 1 yr; n = 16) at rest. A separate group of OM performed a single bout of resistance exercise and additional muscle biopsies were taken 24 and 48 hours post‐exercise; this was performed before and following 12 wks of combined exercise training (OM‐Ex; 73 ± 1; n = 24). Muscle SC niche measurements were assessed using high resolution immunofluorescent confocal microscopy. Type II SC niche laminin thickness was greater in OM (1.86 ± 0.06 µm) as compared to YM (1.55 ± 0.09 µm, P < .05). The percentage of type II‐associated SC that were completely surrounded by laminin was greater in OM (13.6%±4.2%) as compared to YM (3.5%±1.5%; P < .05). In non‐surrounded SC, the proportion of active MyoD+/Pax7+ SC were higher compared to surrounded SC (P < .05) following a single bout of exercise. This “incarceration” of the SC niche by laminin appears with aging and may inhibit SC activation in response to exercise.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    584Downloads
    6 month trend
    149Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record