e-space
Manchester Metropolitan University's Research Repository

    A Modular Bioplatform Based on a Versatile Supramolecular Multienzyme Complex Directly Attached to Graphene

    Alshammari, A, Posner, MG, Upadhyay, A, Marken, F, Bagby, S and Ilie, A (2016) A Modular Bioplatform Based on a Versatile Supramolecular Multienzyme Complex Directly Attached to Graphene. ACS Applied Materials and Interfaces, 8 (32). pp. 21077-21088. ISSN 1944-8244

    [img]
    Preview
    Accepted Version
    Available under License In Copyright.

    Download (1MB) | Preview

    Abstract

    © 2016 American Chemical Society. Developing generic strategies for building adaptable or multifunctional bioplatforms is challenging, in particular because protein immobilization onto surfaces often causes loss of protein function and because multifunctionality usually necessitates specific combinations of heterogeneous elements. Here, we introduce a generic, modular bioplatform construction strategy that uses cage-like supramolecular multienzyme complexes as highly adaptable building blocks immobilized directly and noncovalently on graphene. Thermoplasma acidophilum dihydrolipoyl acyltransferase (E2) supramolecular complexes organize as a monolayer or can be controllably transferred onto graphene, preserving their supramolecular form with specific molecular recognition capability and capacity for engineering multifunctionality. This E2-graphene platform can bind enzymes (here, E1, E2's physiological partner) without loss of enzyme function; in this test case, E1 catalytic activity was detected on E2-graphene over 6 orders of magnitude in substrate concentration. The E2-graphene platform can be multiplexed via patterned cotransfer of differently modified E2 complexes. As the E2 complexes are robust and highly customizable, E2-graphene is a platform onto which multiple functionalities can be built.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    541Downloads
    6 month trend
    190Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record