e-space
Manchester Metropolitan University's Research Repository

Sensitive determination of amlodipine besylate using bare/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids

Khairy, M, Khorshed, AA, Rashwan, FA, Salah, GA, Abdel-Wadood, HM and Banks, CE (2017) Sensitive determination of amlodipine besylate using bare/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids. Sensors and Actuators B: Chemical, 239. pp. 768-775. ISSN 0925-4005

[img]
Preview

Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

The screen-printed technique is widely used as an efficient tool for electrochemical analysis in environment, clinical and agri-food areas. Significantly, it has the ability to transfer electrochemical laboratory experiments into the field. In the present work, we report a highly sensitive, simple, low-cost protocol for determination of amlodipine (AML) using bare/unmodified and DNA-modified screen-printed electrodes (SPEs). The immobilization of DNA molecules onto SPE offers promising robust and chemically stable molecular wires, which provides a unique opportunity for charge transfer processes. Consequently, the electroanalytical sensing of AML was explored at bare/unmodified and DNA-modified SPEs in a linear range between 0.066–1.0 μM and 0.066–2.0 μM with the detection limit (3σ) found to be 20.70 nM and 14.94 nM, whilst corresponding sensitivities of: 0.43 A L mol−1 and 4.23 A L mol−1 respectively. Although, the superior electrochemical signature of bare SPEs is evident, the immobilization of DNA onto SPEs enhances the sensitivity 10-times more than the bare SPEs. Furthermore, the optimized electroanalytical protocol using the unmodified SPEs, which requires no pre-treatment and electrode modification step, was then further applied to the determination of AML in real samples.

Impact and Reach

Statistics

Activity Overview
6 month trend
219Downloads
6 month trend
294Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

View Item View Item