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Maternal High-fat Diet During Pregnancy Programs Platelet Hyperactivation in Male Mouse Offspring

Gaspar, Renato Simões, Unsworth, Amanda J, Bye, Alex, Sage, Tanya, Stewart, Michelle, Wells, Sara, Cox, Roger D, Gibbins, Jonathan M, Sellayah, Dyan and Hughes, Craig (2021) Maternal High-fat Diet During Pregnancy Programs Platelet Hyperactivation in Male Mouse Offspring. Scientific Reports, 11. ISSN 2045-2322

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Maternal over-nutrition increases the risk of diabetes and cardiovascular events in offspring. While prominent effects on cardiovascular health are observed, the impact on platelet physiology has not been studied. Here, we examined whether maternal high-fat diet (HF) ingestion affects the platelet function in lean and obese offspring. C57BL6/N mice dams were given a HF or control (C) diet for 8 weeks before and during pregnancy. Male and female offspring received C or HF diets for 26 weeks. Experimental groups were: C/C, dam and offspring fed standard laboratory diet; C/HF dam fed standard laboratory diet and offspring fed HF diet; HF/C and HF/HF. Phenotypic and metabolic tests were performed and blood collected for platelet studies. Compared to C/C, offspring HF groups were obese, with fat accumulation, hyperglycaemia and insulin resistance. Female offspring did not present platelet hyperactivity, hence we focused on male offspring. Platelets from HF/HF mice were larger, hyperactive and presented oxidative stress when compared to C/C. Maternal and offspring HF diet results in platelet hyperactivation in male mouse offspring, suggesting a novel ‘double-hit’ effect.

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