e-space
Manchester Metropolitan University's Research Repository

    Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells

    Poliandri, A, Miller, D, Howard, S, Nobles, M, Ruiz-Babot, G, Harmer, S, Tinker, A, McKay, T, Guasti, L and Dunkel, L (2017) Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells. Molecular and Cellular Endocrinology, 447. pp. 12-22. ISSN 0303-7207

    [img]
    Preview

    Available under License Creative Commons Attribution Non-commercial No Derivatives.

    Download (371kB) | Preview

    Abstract

    © 2017 Elsevier B.V. GnRH neurons are fundamental for reproduction in all vertebrates, integrating all reproductive inputs. The inaccessibility of human GnRH-neurons has been a major impediment to studying the central control of reproduction and its disorders. Here, we report the efficient generation of kisspeptin responsive GnRH-secreting neurons by directed differentiation of human Embryonic Stem Cells and induced-Pluripotent Stem Cells derived from a Kallman Syndrome patient and a healthy family member. The protocol involves the generation of intermediate Neural Progenitor Cells (NPCs) through long-term Bone morphogenetic protein 4 inhibition, followed by terminal specification of these NPCs in media containing Fibroblast Growth Factor 8 and a NOTCH inhibitor. The resulting GnRH-expressing and -secreting neurons display a neuroendocrine gene expression pattern and present spontaneous calcium transients that can be stimulated by kisspeptin. These in vitro generated GnRH expressing cells provide a new resource for studying the molecular mechanisms underlying the development and function of GnRH neurons.

    Impact and Reach

    Statistics

    Activity Overview
    6 month trend
    488Downloads
    6 month trend
    413Hits

    Additional statistics for this dataset are available via IRStats2.

    Altmetric

    Repository staff only

    Edit record Edit record