e-space
Manchester Metropolitan University's Research Repository

Effect of GH on human skeletal muscle lipid metabolism in GH deficiency.

Trepp, Roman and Flüeck, Martin and Stettler, Christoph and Boesch, Chris and Ith, Michael and Kreis, Roland and Hoppeler, Hans and Howald, Hans and Schmid, Jean-Paul and Diem, Peter and Christ, Emanuel R. (2008) Effect of GH on human skeletal muscle lipid metabolism in GH deficiency. American journal of physiology. Endocrinology and metabolism, 294 (6). E1127-E1134. ISSN 1522-1555

Full text not available from this repository.

Abstract

Adult-onset growth hormone (GH) deficiency (GHD) is associated with insulin resistance and decreased exercise capacity. Intramyocellular lipids (IMCL) depend on training status, diet, and insulin sensitivity. Using magnetic resonance spectroscopy, we studied IMCL content following physical activity (IMCL-depleted) and high-fat diet (IMCL-repleted) in 15 patients with GHD before and after 4 mo of GH replacement therapy (GHRT) and in 11 healthy control subjects. Measurements of insulin resistance and exercise capacity were performed and skeletal muscle biopsies were carried out to assess expression of mRNA of key enzymes involved in skeletal muscle lipid metabolism by real-time PCR and ultrastructure by electron microscopy. Compared with control subjects, patients with GHD showed significantly higher difference between IMCL-depleted and IMCL-repleted. GHRT resulted in an increase in skeletal muscle mRNA expression of IGF-I, hormone-sensitive lipase, and a tendency for an increase in fatty acid binding protein-3. Electron microscopy examination did not reveal significant differences after GHRT. In conclusion, variation of IMCL may be increased in patients with GHD compared with healthy control subjects. Qualitative changes within the skeletal muscle (i.e., an increase in free fatty acids availability from systemic and/or local sources) may contribute to the increase in insulin resistance and possibly to the improvement of exercise capacity after GHRT. The upregulation of IGF-I mRNA suggests a paracrine/autocrine role of IGF-I on skeletal muscle.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
156Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item