e-space
Manchester Metropolitan University's Research Repository

Investigation of alpha nascent polypeptide-associated complex functions in a human CD8+ T cell ex vivo expansion model using antisense oligonucleotides

Al-Shanti, Nasser and Steward, C. G. and Garland, R. J. and Rowbottom, A. W. (2004) Investigation of alpha nascent polypeptide-associated complex functions in a human CD8+ T cell ex vivo expansion model using antisense oligonucleotides. Immunology, 112 (3). pp. 397-403. ISSN 1365-2567

Full text not available from this repository.

Abstract

In order to determine molecules involved in the differentiation and proliferation of human CD8+ cells, two ex vivo expansion models were established: coculture of freshly purified human CD8+ cells with irradiated autologous feeders (AF) or stimulation with anti-CD3. Two different proliferation kinetics of CD8+ cells and expression patterns of CD57 were observed between these conditions. Differential display reverse transcriptase–polymerase chain reaction was applied to investigate the differential expression of mRNA species between CD8+ CD57+ and CD8+ CD57– populations. A differentially expressed RNA species called alpha nascent polypeptide associated complex (α NAC) was found at a higher level in CD8+ CD57– cells than in CD8+ CD57+ cells. In the presence of AF, the expression of α NAC was reduced on culturing whilst proliferation increased. Similarly, in cultures stimulated with anti-CD3, α NAC reverted to its inactive form and differentiation and proliferation increased. Using a phosphorothioate-modified oligodeoxynucleotide antisense directed specifically against α NAC mRNA, protein expression was inhibited and increased CD8+ cell proliferation and CD25 expression were observed irrespective of the culture conditions. This suggests that α NAC protein is antiproliferative molecule. This is the first description of the function of the α NAC protein in human CD8+ T cells.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
121Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item