e-space
Manchester Metropolitan University's Research Repository

Effect of dextranase and protease enzymes on aggregation of Streptococcus mutans: colorimetric and electron microscopic studies.

Graves, W. and Verran, Joanna (1984) Effect of dextranase and protease enzymes on aggregation of Streptococcus mutans: colorimetric and electron microscopic studies. ISSN 0026-2633

Full text not available from this repository.

Abstract

Sucrose-induced aggregation of Streptococcus mutans was ascertained by measuring the decrease in optical density of a cell suspension after 20 h incubation. The optimum sucrose concentration for the aggregation assay was 5% w/v, at an incubation temperature of 37 degrees C. Dextranase and protease enzymes were added to the suspensions to determine their effects on aggregation. Dextranase treatment decreased aggregation. Electron microscopic studies revealed a reduction in the amount of a diffuse coat which surrounded cells in the absence of the enzyme but in the presence of sucrose, and a degradative effect on fibrous extracellular material. Protease treatment of suspensions produced an increase in optical density; cell aggregates were not apparent. Electron microscopic studies revealed extensive cell damage and lysis. The success of these enzymes in decreasing aggregation would indicate a role in both caries and plaque control in vivo.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
62Hits

Additional statistics for this dataset are available via IRStats2.

Actions (login required)

Edit Item Edit Item