e-space
Manchester Metropolitan University's Research Repository

EphB2 regulates contact-dependent and contact-independent signaling to control platelet function

Vaiyapuri, Sakthivel and Sage, Tanya and Rana, Rekha H and Schenk, Michael P and Ali, Marfoua S and Unsworth, Amanda J and Jones, Chris I and Stainer, Alexander R and Kriek, Neline and Moraes, Leonardo A and Gibbins, Jonathan M (2015) EphB2 regulates contact-dependent and contact-independent signaling to control platelet function. Blood, 125 (4). pp. 720-730. ISSN 0006-4971

[img]
Preview

Download (2MB) | Preview

Abstract

The Eph kinases, EphA4 and EphB1, and their ligand, ephrinB1, have been previously reported to be present in platelets where they contribute to thrombus stability. Although thrombus formation allows for Eph-ephrin engagement and bidirectional signaling, the importance specifically of Eph kinase or ephrin signaling in regulating platelet function remained unidentified. In the present study, a genetic approach was used in mice to establish the contribution of signaling orchestrated by the cytoplasmic domain of EphB2 (a newly discovered Eph kinase in platelets) in platelet activation and thrombus formation. We conclude that EphB2 signaling is involved in the regulation of thrombus formation and clot retraction. Furthermore, the cytoplasmic tail of this Eph kinase regulates initial platelet activation in a contact-independent manner in the absence of Eph-ephrin ligation between platelets. Together, these data demonstrate that EphB2 signaling not only modulates platelet function within a thrombus but is also involved in the regulation of the function of isolated platelets in a contact-independent manner.

Impact and Reach

Statistics

Downloads
Activity Overview
18Downloads
52Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item