e-space
Manchester Metropolitan University's Research Repository

p17 from HIV induces brain endothelial cell angiogenesis through EGFR-1-mediated cell signalling activation

Liu, D and Zeinolabediny, Y and Caccuri, F and Ferris, G and Fang, WH and Weston, R and Krupinski, J and Colombo, L and Salmona, M and Corpas, R and Sarroca, S and Sanfeliu, C and Caruso, A and Guo, B and Zeng, X and Slevin, M (2018) p17 from HIV induces brain endothelial cell angiogenesis through EGFR-1-mediated cell signalling activation. Laboratory Investigation. ISSN 0023-6837

[img]
Restricted to Repository staff only until 2 June 2019.

Download (76kB)

Abstract

© 2018, United States & Canadian Academy of Pathology. HIV-associated neurocognitive disorder in HIV patients substantially reduces their quality of life. We previously showed that the HIV matrix protein, p17 could stimulate lymph-angiogenesis in vitro potentially contributing to lymphoma tumour growth and in addition is associated with vascular activation in neuro-degenerating brain tissue; here, therefore, we have investigated the detailed molecular mechanisms of this action. We performed in vitro cell culture, angiogenesis experiments, phospho-protein microarrays and Western blotting to identify cellular signalling induced by p17 within human brain endothelial cells (HbMEC), and inhibitor studies to block p17-induced vascular growth. We also characterised the effects of hippocampal CA1 injection of p17 on epidermal growth factor receptor-1 (EGFR1) expression linked to our murine model of dementia. p17 strongly induced angiogenesis of HbMEC (migration, tube formation and spheroid growth). p17 concomitantly increased phosphorylation of EGFR1 as well as down-stream intermediates ERK1/2, FAK, PLC-γ and PKC-β whilst an inhibitor peptide of EGFR, blocked cell signalling and angiogenesis. Finally, Mice that showed reduced cognitive function and behavioural deficiencies after p17 injection, demonstrated that p17 localised in cortical microvessels and also neurones many of which stained positive for p-EGFR1 by histology/IHC. This work provides strong support that p17 may be involved in initiating and/or perpetuating vascular tissue pathophysiology associated with comorbidity in HIV patients.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
0Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item