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Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide

Velusamy, V and Palanisamy, S and Chen, SM and Chen, TW and Selvam, S and Ramaraj, SK and Lou, BS (2017) Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide. Sensors and Actuators, B: Chemical, 252. pp. 175-182. ISSN 0925-4005

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Abstract

© 2017 Elsevier B.V. In the present work, we have investigated the electrochemical behavior and electrocatalysis of hemoglobin (Hb) immobilized on a glassy carbon electrode (GCE) modified with a graphene-cellulose microfiber (GR–CMF) composite. The GR–CMF composite was characterized by scanning electron microscopy, elemental analysis, and Raman and Fourier transform infrared spectroscopy. Well-defined electrochemical redox characteristics of Hb were observed for Hb immobilized on a GR–CMF composite modified GCE, with a formal potential of −0.306V and a peak to peak separation of approximately 67 mV. Due to the high biocompatibility of the GR–CMF composite, the electrochemical behavior of the Hb heme redox couple (FeII/FeIII) was enhanced for Hb immobilized on the GR–CMF composite when compared to Hb immobilized on pristine GR. The heterogeneous electron transfer constant (ks) was calculated as 6.17 s−1, and is higher than previously reported for Hb immobilized GR supports. The Hb immobilized GR–CMF composite modified electrode was used for the quantification of H2O2 under optimal conditions, and shows a wider linear amperometric response ranging from 0.05 to 926 M. The limit of detection of the biosensor was 0.01 M with the sensitivity of 0.49 A M−1 cm−2. The biosensor also showed high selectivity in the presence of the range of interfering compounds and exhibits good operational stability and practicality in the detection of H2O2.

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