e-space
Manchester Metropolitan University's Research Repository

Effect of surface conditioning with cellular extracts on Escherichia coli adhesion and initial biofilm formation

Moreira, JMR and Gomes, LC and Whitehead, KA and Lynch, S and Tetlow, LA and Mergulhão, FJ (2017) Effect of surface conditioning with cellular extracts on Escherichia coli adhesion and initial biofilm formation. Food and Bioproducts Processing, 104. pp. 1-12. ISSN 0960-3085

[img]
Preview

Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Preview

Abstract

Bacterial adhesion and subsequent biofilm formation start with surface conditioning by molecules originating from the surrounding medium and from cell lysis. Different cell extracts e.g. total cell extract (TCE), cytoplasm with cellular debris (CCDE) and periplasmic extract (PE) were tested in agitated 96-well microtiter plates and in a flow cell. Crystal violet assay demonstrated that a polystyrene substratum conditioned with TCE or CCDE decreased initial biofilm formation, however cell adhesion generally increased when PE was used. These results were dependent on conditioning film concentration. Using a parallel plate flow chamber, the use of optimal conditioning film concentrations resulted in all the different cellular extracts reducing biofilm formation. Multifractal analysis was used to generate quantitative data on the number of cell clusters. Surface conditioning with cellular components affected the amount and clustering of bacteria on polystyrene surfaces and their propensity to induce biofilm formation. To the best of our knowledge, this is the first study addressing the effect of cellular surface conditioning of cellular compartments on E. coli adhesion and initial biofilm formation. This work leads to a greater understanding of the factors that influence biofilm formation under flow conditions which are prevalent in food industry.

Impact and Reach

Statistics

Downloads
Activity Overview
16Downloads
71Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item