Sex identification of ancient DNA samples using a microfluidic device.

Shaw, KJ, Brown, KA, Brown, TA and Haswell, SJ (2015) Sex identification of ancient DNA samples using a microfluidic device. Methods in Molecular Biology, 1274.

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Abstract

Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidin-coated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker.

Item Type:	Article
Peer-reviewed:	No
Date Deposited:	21 Jun 2016 14:18
Publisher:	Springer
Additional Information:	This is an Author Final Copy of a paper accepted for publication in Methods in Molecular Biology, published by and copyright Springer.
Divisions:	Faculties > Science and Engineering Research Centres > Ecology and Environment Research Centre
URI:	https://e-space.mmu.ac.uk/id/eprint/613848
DOI:	https://doi.org/10.1007/978-1-4939-2353-3_8

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