e-space
Manchester Metropolitan University's Research Repository

The cooperative transforming effects of PAX3-FKHR and IGF-II on mouse myoblasts

Slevin, Mark and Parker, Craig J. and Kumar, Patricia and Wang, Weizhi (2005) The cooperative transforming effects of PAX3-FKHR and IGF-II on mouse myoblasts. ISSN 1019-6439

Full text not available from this repository.

Abstract

Alveolar rhabdomyosarcoma (ARMS) cells express high levels of PAX3-FKHR and IGF-II. In this study, we have investigated the effects of PAX3-FKHR and IGF-II on the expression of muscle regulatory factors (myf5, MyoD and myogenin), and platelet derived growth factor-B (PDGF-B) and vascular endothelial growth factor (VEGF) in mouse C2C12 myoblasts in vitro. PAX3-FKHR induced cell cycling of C2C12 cells and promoted proliferation whilst blocking myogenesis. IGF-II inhibited their differentiation without influencing proliferation. Western blotting showed that PAX3-FKHR and IGF-II blocked the expression of myogenin and MyoD respectively. Since MyoD affects early myogenesis and myogenin controls terminal differentiation, a combination of PAX3-FKHR and IGF-II synergistically blocks myogenesis at several different stages in differentiation. We have also shown that the major survival and angiogenic cytokines, PDGF-B and VEGF, were induced by IGF-II and PAX3- FKHR respectively. A combination of PAX3-FKHR and IGF-II could synergistically up regulate the expression of PDGF-B and VEGF and stabilize their high expression levels. Our results suggest that high expression of PAX3-FKHR and IGF-II in ARMS synergistically play a key role in oncogenesis and tumour progression of ARMS.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
65Hits

Additional statistics for this dataset are available via IRStats2.

Actions (login required)

Edit Item Edit Item