e-space
Manchester Metropolitan University's Research Repository

Use of the atomic force microscope to determine the effect of substratum surface topography on bacterial adhesion

Boyd, Robert D. and Verran, Joanna and Jones, M. V. and Bhakoo, Manmohan (2002) Use of the atomic force microscope to determine the effect of substratum surface topography on bacterial adhesion. Langmuir, 18 (6). pp. 2343-2346. ISSN 0743-7463

Full text not available from this repository.

Abstract

Changes in surface roughness and topography on the macroscopic scale are known to affect bacterial attachment and retention. Little quantitative information is available as to how changes in surface topography on the micron and submicron scale affect the strength of bacterial attachment to substrata. A novel method is described using the atomic force microscope where a varying shear/lateral force (in nanonewtons) is used to detach individual bacterial cells from various substrata of different surface topographies. Lateral changes of 0.1 μm in the surface topography are sufficient to affect the strength of bacterial attachment. An increase in applied force from 4 to 8 nN was necessary to move bacteria retained in surface defects of approximately 1 μm wide and 0.2 μm deep compared with cells attached on smooth surfaces.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
65Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item