e-space
Manchester Metropolitan University's Research Repository

The time course of denervation-induced changes is similar in soleus muscles of adult and old rats

Degens, Hans and Koşar, Sükran Nazan and Hopman, Maria T.E. and Haan, Arnold de (2008) The time course of denervation-induced changes is similar in soleus muscles of adult and old rats. nutrition and metabolism, 33 (2). pp. 299-308. ISSN 1715-5312

Full text not available from this repository.

Abstract

Muscle denervation is accompanied by atrophy and a decline in oxidative capacity. We investigated whether the time course of adaptations following denervation of the soleus muscle differs in adult (5 months old) and older adult (25 months old) rats. We denervated the soleus muscle of the left leg, while the right leg served as an internal control. Two weeks after denervation, muscle mass was decreased both in adult and old animals to, respectively, 57% and 54% (p < 0.001) and capillary to fibre ratio (C:F) decreased to 51% and 50% (p < 0.01) of the control values. Yet, the capillary density was increased in older adult but not in adult muscles, indicating that the regression of the capillary bed during denervation lags behind the decrease in fibre size in the soleus muscle of the older rats. One week after denervation the optical density of sections stained for succinate dehydrogenase was 83% and 79% (p < 0.05) of control adult and older adult muscles, respectively, and then remained stable. This indicates that during the first week of denervation loss of oxidative capacity occurred at a relatively higher rate than that of muscle mass. No major changes occurred between 2 and 4 weeks of denervation, except for an increase in the proportion of hybrid (I/IIa) fibres in 4 week denervated muscles (adult 10% vs. 23%; old 1% vs. 13%; p < 0.05). Except for changes in capillarisation, the time course of atrophy and decrease in oxidative capacity following denervation was similar in soleus muscles from adult and old rats.

Impact and Reach

Statistics

Downloads
Activity Overview
0Downloads
65Hits

Additional statistics for this dataset are available via IRStats2.

Altmetric

Actions (login required)

Edit Item Edit Item