Rajan, Senthilkumar S. and Balasubramaniem, Ashokkumar and Krishnan, Chandraraj and Dempsey, Michael J. and Paramasamy, Gunasekaran (2005) Thermal inactivation of alkali stable xylanases (XylA & XylB) from alkali tolerant fungus Aspergillus fischeri Fxn 1: effect of trehalose on their thermostability. In: UNSPECIFIED.Full text not available from this repository.
Alkali tolerant fungus Aspergillus fischeri Fxn 1 secretes two xylanases, an exoxylanase (XylA) and an endoxylanase (XylB) in solid state fermentation. Kinetic studies showed that thermal inactivation of purified XylA and XylB at pH 9, in the temperature ranging from 303K to 333K, followed first-order kinetics, with denaturation rate constants as 9.6 10-3 and 8.2 10-3 at 323K for XylA & XylB respectively. Heat-inactivation plots for purified enzymes were linear from which thermodynamic activation parameters, ΔH#, ΔS# and ΔG# have been estimated. The enzymatic activity was relatively stable with a respective half-life (t1/2) at 323K of 72 min for XylA and 84.5 min for XylB. The half life values of XylA & XylB increased to 169 and 136 min respectively, in the presence of 1 M trehalose. Separate tests at 60 C in the presence of additives (polyols, carbohydrates) showed that trehalose was the most effective stabiliser, which increased the stability of XylA & XylB to 64.7 & 17.63 % respectively.
|Item Type:||Conference or Workshop Item (Paper)|
|Additional Information:||Presented at Bioscience 2005 (Biochemical Society meeting), Glasgow, July 17-21, 2005.|
|Divisions:||Faculties > Faculty of Science and Engineering > Department of Biological Sciences Research: Fermentation and Industrial Microbiology (Biotechnology)|
|Date Deposited:||22 Aug 2007 09:32|
|Last Modified:||15 Sep 2016 13:45|
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