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Detection of Konjac glucomannan by immunoassay

Hurley, Ian P. and Pickles, Neil A. and Qin, Hongmei and Elyse Ireland, H. and Coleman, Robert C. and Tosun, Berat N. and Buyuktuncer, Zehra and Williams, John H. H. (2010) Detection of Konjac glucomannan by immunoassay. ISSN 1365-2621

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Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL−1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.

Item Type: Article
Additional Information: Full-text of this article is not available in this e-prints service. This article was originally published following peer-review in International Journal of Food Science and Technology, published by and copyright Wiley-Blackwell Publishing Ltd.
Divisions: Faculties > Hollings Faculty > Food, Nutrition and Hospitality
Date Deposited: 02 Sep 2010 13:02
Last Modified: 01 Sep 2016 14:02
URI: http://e-space.mmu.ac.uk/id/eprint/110623


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